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KMID : 0978920000010040051
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Korean Journal of Clinical Geriatrics
2000 Volume.1 No. 4 p.51 ~ p.62
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A Study on some polymorphisms of human leptin receptor gene in Koreans
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Choi Bong-Keun
Yoon Tai-Young
Choi Joon-Myung
Park Soon-Young
Lew Dong-Joon
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Abstract
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lnstoduction: Leptin is an adipocyte-derived blood borne satiety factor that acts on its cognate leptin receptor(Ob-R) in the hypothalamus, thereby regrulating food intake and energy expenditure. To exlore whether mutations in the Ob-R gene cause obesity in humans, we have searched for mutations in the gene for Ob-Rb, a biologically active receptor isofonn, in obese korean subjects.
Metheods: Genomic DNA was extracted from whole blo여 stored at the time of colletion, and used for subsequent analyses. PCR-RFLP analysis was performed for genot째,ing at codons 2Z3 and 1019.
Result: : In obese subjects, allele frequencies of the polymorphic site at the codons 2Z3 and 1019 (Gln223Arg and Prol019pro) were 91 and Erl%. In non-obese subjests, they wre 92 and 86%. The odds ratio (95% confidence interval) for obesity of Arg223 and prol019pro allele were 0.9 (0.3-2.6) and 1.1 (0.5-2.5) , respectively. No significant 이fference was observed in the clinical characteristics among the genotype groui:s. Diastolic blood pressure of subjects with he Gln/Gln and Gln/ Arg in the non -obese. There are no significant 띠fference in TC, TG, HDL-C and LDL-C among the Ob-R genotype groups.
Conclusion: Diastolic blood pressure of subjects with the Gln/Gln and Gln/ Arg in the obese was significantly
higher than that of those with Gln/Gln and Gln/ Arg in the non -obese. Hypertension is associated with obesity. Further studies are requird to clarity the association 양tween polymorphism of Ob-R gene and hypertension.
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KEYWORD
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Obesity, Reptin receptor, PCR-RFLP, polymorphism, Diastolic pressure, Hypertension
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